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The following cell lines were used: CT26, a carcinogen-induced, undifferentiated colon carcinoma obtained from BALB/c mice (51); TS/A, a mouse mammary adenocarcinoma derived from BALB/c mice (52); MCA203, a C57BL/6-derived fibrosarcoma (53); and B16-GM, a C57BL/6 melanoma cell line genetically modified to secrete GM-CSF (54). The 4T1-HA cell line was obtained by lentiviral transduction of 4T1 mammary carcinoma and was provided by K. Whartenby (Johns Hopkins University, Baltimore, MD). These cell lines were grown in DMEM or RPMI 1640 (Invitrogen) with 10% FBS (Invitrogen). The C26GM cell line derived from the C26 colon carcinoma was genetically modified to produce GM-CSF (8) and was grown in the presence of 800 µg/ml G418. Your browser's settings may be preventing you from commenting on and viewing comments about this item. See instructions for fixing the problem. This focus on performance, Shaw says, robs men and their partners of the opportunity to deepen their relationships -- and their sexuality. The sexual needs of adolescents, she says, are different that those of adults. Yet by making erections the be-all and end-all of sex, grown men are retreating to adolescent sexual values. "We as physicians should be asking about, and men should be reporting to their physicians, symptoms of ED, so it can be considered as we work to modify their risk -- treat blood pressure, cholesterol more aggressively, advise healthy lifestyle changes like exercise and healthy diet," he said. Q. What are the major differences between the two devices? Viagra online